All Journals > The Journal of Infectious Diseases > 1 April 2008 > Inflammation and C. albicans Colonization in Mice

Article Tools

Search for Related Articles

  • By Author
  • Search In

Announcements

Science Watch logo

JID Article Named "New Hot Paper" by ScienceWatch.com

Dr. Lauri Hicks' 2007 article on pneumococcal disease has been named a "hot new paper" by Thompson Reuters' ScienceWatch.com. Read a Q&A about the article with Dr. Hicks here. 

Press Release

Unique Collaboration Charts the Migrations of a Parasite that Affected History
Researchers Sequence Louse DNA from Mummies and Propose New Model for its Development


In the News

Featured in Grist
"Another symptom of swine flu: instant amnesia" May 11, 2009
Swine Influenza Virus: Zoonotic Potential and Vaccination Strategies for the Control of Avian and Swine Influenzas
Eileen Thacker and Bruce Janke
Read the veterinary literature on swine flu and you get a strong sense of what might be called vaccination treadmill: the hog industry is literally scrambling to generate new vaccines for the rapidly evolving flu strains that sweep through CAFOs. Writing in the Journal of Infectious Diseases [PDF] in 2008, Eileen Thacker and Bruce Janke of Iowa State University paint a stark picture: “A number of genetically diverse viruses are circulating in swine herds throughout the world and are a major cause of concern to the swine industry,” they write. “Influenza virus infections in swine and poultry are potential sources of viruses for the next pandemic among humans.”

Featured in New York Times
"Fear of a Swine Flu Epidemic in 1976 Offers Some Lessons, and Concerns, Today" May 8, 2009
Anti‐Ganglioside Antibody Induction by Swine (A/NJ/1976/H1N1) and Other Influenza Vaccines: Insights into Vaccine‐Associated Guillain‐Barré Syndrome
Irving Nachamkin, Sean V. Shadomy, Anthony P. Moran, Nancy Cox, Collette Fitzgerald, Huong Ung, Adrian T. Corcoran, John K. Iskander, Lawrence B. Schonberger, and Robert T. Chen
Irving Nachamkin, a professor of pathology and laboratory medicine at the University of Pennsylvania, examined some 1976 vaccine that had been saved by a scientist in Texas. In a paper published last year in The Journal of Infectious Diseases, he and colleagues reported that mice given the vaccine made antibodies that reacted with gangliosides, which are components of nerve cells. An antibody attack on gangliosides is part of the disease mechanism of Guillain-Barré.

Featured in AFP
"Swine flu vaccine 'could be ready soon'" May 7, 2009
A Broadly Protective Vaccine against Globally Dispersed Clade 1 and Clade 2 H5N1 Influenza Viruses
Mary A. Hoelscher, Neetu Singh, Sanjay Garg, Lakshmi Jayashankar, Vic Veguilla, Aseem Pandey, Yumi Matsuoka, Jacqueline M. Katz, Ruben Donis, Suresh K. Mittal, and Suryaprakash Sambhara
The vaccine Mittal created for the bird flu worked on three different strains isolated over a seven-year period and was described in papers for the Journal of Infectious Diseases and the journal Clinical Pharmacology and Therapeutics.

Featured in Newsweek
"The Path of a Pandemic" http://www.newsweek.com/id/195692
Swine Influenza Virus: Zoonotic Potential and Vaccination Strategies for the Control of Avian and Swine Influenzas
Eileen Thacker and Bruce Janke
Last year researchers from Iowa State University in Ames warned that pigs located in industrial-scale farms were being subjected to influenza infections from farm poultry, wild birds and their human handlers. Writing in The Journal of Infectious Diseases, Eileen Thacker and Bruce Janke said, "As a result of the constantly changing genetic makeup of individual influenza viruses in pigs, the U.S. swine industry is continually scrambling to respond to the influenza viruses circulating within individual production systems."

1 April 2008

Volume 197, Number 7
The Journal of Infectious Diseases 2008;197:972–980
© 2008 by the Infectious Diseases Society of America. All rights reserved.
0022-1899/2008/19707-0008$15.00
DOI: 10.1086/528990
MAJOR ARTICLE

Colonization of Mice by Candida albicans Is Promoted by Chemically Induced Colitis and Augments Inflammatory Responses through Galectin‐3

Samir Jawhara,1,3

Xavier Thuru,3

Annie Standaert‐Vitse,1,2

Thierry Jouault,1

Serge Mordon,2

Boualem Sendid,1,5

Pierre Desreumaux,3 and

Daniel Poulain1,5

1Inserm U 799, Physiopathologie des Candidoses, Faculté de Médecine, Centre Hospitalier Régional Universitaire de Lille, Institut Fédératif de Recherche 114, Université Lille 2, 2Laboratoire de Parasitologie, Faculté de Pharmacie, Avenue du Professeur Laguesse, 3Inserm U 795, Physiopathologie des Maladies Inflammatoires Intestinales, 4UPRES EA 2689, Détresses Respiratoires et Circulatoires, Pavillon Vancostenobel 5Laboratoire de Parasitologie‐Mycologie, Pôle de Microbiologie, Centre Hospitalier Universitaire, Lille, France

Background.Little is known about the relationship between colonic inflammation and Candida albicans colonization. Galectin‐3 (Gal‐3) is an intestinal lectin that binds to specific C. albicans glycans and is involved in inflammation.

Methods.Colitis was experimentally induced in wild‐type and Gal3−/− mice using dextran sulfate sodium (DSS) before oral administration of C. albicans. Yeast recovered from stools was quantified. The presence of yeast and inflammation were evaluated in sections of colon by histologic examination, quantification of myeloperoxidase (MPO) activity, and by gene expression for cytokines and innate immune receptors. Serum from mice was collected for determination of anti‐yeast mannan antibodies, including anti–Saccharomyces cerevisiae antibodies (ASCA), which are biomarkers of an inflammatory bowel disease.

Results.Inflammation strongly promoted C. albicans colonization. Conversely, C. albicans augmented inflammation induced by DSS, as assessed by histologic scores, MPO activity, and tumor necrosis factor (TNF)‐α and Toll‐like receptor (TLR)‐2 expression. C. albicans colonization generated ASCA. The absence of Gal‐3 reduced DSS inflammation and abolished the response of TLR‐2 and TNF‐α to C. albicans colonization.

Conclusions.DSS‐induced colitis provides a model for establishing C. albicans colonization in mice. This model reveals that C. albicans augments inflammation and confirms the role of Gal‐3 in both inflammation and the control of host responses to C. albicans.

Received 4 May 2007; accepted 4 October 2007; electronically published 4 March 2008.

Reprints or correspondence: Daniel Poulain, Inserm U799, Physiopathologie des Candidoses, Faculté de Médecine, 1, Place de Verdun, 59045 Lille Cedex, France ().

Cited by

Annie Standaert-Vitse, Boualem Sendid, Marie Joossens, Nadine François, Peggy Vandewalle-El Khoury, Julien Branche, Herbert Van Kruiningen, Thierry Jouault, Paul Rutgeerts, Corinne Gower-Rousseau, Christian Libersa, Christel Neut, Franck Broly, Mathias Chamaillard, Séverine Vermeire, Daniel Poulain, Jean-Frédéric Colombel. (2009) Candida albicans Colonization and ASCA in Familial Crohn's Disease. The American Journal of Gastroenterology 104:7, 1745-1753
Online publication date: 1-Aug-2009.
CrossRef
Gerardo R. Vasta. (2009) Roles of galectins in infection. Nature Reviews Microbiology 7:6, 424-438
Online publication date: 1-Jul-2009.
CrossRef
Guillaume Pineton de Chambrun, Jean-Frédéric Colombel, Daniel Poulain, Arlette Darfeuille-Michaud. (2008) Pathogenic agents in inflammatory bowel diseases. Current Opinion in Gastroenterology 24:4, 440-447
Online publication date: 1-Aug-2008.
CrossRef

  • Presented in part: 16th congress of the International Society for Human and Animal Mycology (ISHAM), 25–29 June 2006, Paris, France (abstract 0-0004).

    Potential conflicts of interest: none reported.

    Financial support: Institut National de la Santé et de la Recherche Médicale (Inserm), Région Nord Pas de Calais— FEDER (R06042EE), Région Nord Pas de Calais, France (03530111 to S.J.).

Back to Top
Close Popup