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15 August 2007

Volume 196, Number 4
The Journal of Infectious Diseases 2007;196:528–536
© 2007 by the Infectious Diseases Society of America. All rights reserved.
0022-1899/2007/19604-0007$15.00
DOI: 10.1086/519691
MAJOR ARTICLE

Early Evolution of Hepatitis C Virus (HCV) Quasispecies after Liver Transplant for HCV‐Related Disease

Evelyne Schvoerer,1,4,5

Eric Soulier,4

Cathy Royer,4

Anne‐Catherine Renaudin,1

Christine Thumann,4

Samira Fafi‐Kremer,1,4,5

Nicolas Brignon,4

Stéphane Doridot,4

Nicolas Meyer,2

Patricia Pinson,6

Bernard Ellero,3

Marie‐Lorraine Woehl‐Jaegle,3

Carole Meyer,3

Philippe Wolf,3

Pierre Zachary,1

Thomas Baumert,4,5 and

Françoise Stoll‐Keller1,4,5

1Laboratoire de Virologie and 2Département d’Information Médicale, Hôpital Civil, and 3Centre de Chirurgie Viscérale et de Transplantation, Hôpital de Hautepierre, Centre Hospitalier Régional Universitaire de Strasbourg, 4Unité INSERM 748, and 5Université Louis Pasteur, Faculté de Médecine, Strasbourg, and 6Centre Hospitalier Universitaire de Bordeaux, Laboratoire de Virologie Hôpital Pellegrin, Bordeaux, France

Background.End‐stage liver disease as a result of chronic hepatitis C virus (HCV) infection is the main indication for liver transplant (LT), but allografts are systematically infected with HCV soon after transplant. Viral quasispecies are poorly described during the early posttransplant period.

Methods.For 17 patients who received an LT for HCV disease, plasma viral quasispecies evolution was determined by sequence analysis of hypervariable region 1 of the E2 envelope gene before transplant (BT), after 7 days (D7), and after 1 month (M1). T helper (Th)1/Th2 cytokine levels were determined concomitantly.

Results.HCV quasispecies showed a significant decrease in amino acid diversity at D7 and M1, compared with BT ( ). A correlation was observed between low plasma tumor necrosis factor–α levels at D7 and decreased quasispecies amino acid complexity at the same date. Nucleic acid diversity was lower for genotype 1 than for genotype 3 infection ( ). The complexity and diversity of amino acids were lower in patients with hepatocellular carcinoma (HCC) BT than in those without HCC ( ). Conserved amino acid residues within quasispecies were shared by the whole cohort before and after LT.

Conclusion.Viral structural and/or host immunological features could favor the emergence of fitter HCV strains after LT.

Received 27 November 2006; accepted 21 February 2007; electronically published 29 June 2007.

Reprints or correspondence: Dr. Evelyne Schvoerer, Institut de Virologie, 3 Rue Koeberlé, 67000 Strasbourg, France ().

Cited by

I. M. V. G. C. Mello, C. Thumann, E. Schvoerer, E. Soulier, J. R. R. Pinho, D. A. M. M. Silvestre, A. T. L. Queiroz, P. Wolf, T. F. Baumert, F. S. Keller, C. A. Pereira. (2009) Conservation of hepatitis C virus nonstructural protein 3 amino acid sequence in viral isolates during liver transplantation. Journal of Viral Hepatitis
Online publication date: 1-Apr-2009.
CrossRef
Frédéric Schramm, Eric Soulier, Cathy Royer, Thierry Weitten, Samira Fafi‐Kremer, Nicolas Brignon, Nicolas Meyer, Bernard Ellero, Marie‐Lorraine Woehl‐Jaegle, Carole Meyer, Philippe Wolf, Michel Doffoël, Thomas F. Baumert, Françoise Stoll‐Keller, and Evelyne Schvoerer. (2008) Frequent Compartmentalization of Hepatitis C Virus with Leukocyte‐Related Amino Acids in the Setting of Liver Transplantation. The Journal of Infectious Diseases 198:11, 1656-1666
Online publication date: 1-Dec-2008.
Abstract-Full Text-PDF Version (891 kB)
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Online publication date: 1-Aug-2008.
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  • Potential conflicts of interest: none reported.

    Financial support: Cellule d’Essais Cliniques des Hôpitaux Universitaires de Strasbourg; CHRU Strasbourg (Projet Hospitalier de Recherche Clinique 2864 et Appel à Projets Internes).

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