Evaluation of Real‐Time Polymerase Chain Reaction for the Detection of Methicillin‐Resistant Staphylococcus aureus on Environmental Surfaces
From Bioquell (UK), Andover, Hampshire, United Kingdom (J.A.O.); the Hospital of Saint Raphael (N.L.H., J.M.B.) and the Yale University School of Medicine (J.M.B.), New Haven, Connecticut.
We compared real‐time polymerase chain reaction (RT‐PCR) with in vitro culture for detecting methicillin‐resistant Staphylococcus aureus in samples from environmental surfaces. The sensitivity of RT‐PCR, compared with culture, was 92.5%, and the specificity was 51.4%. Because of poor specificity, the RT‐PCR kit tested is not suitable for the detection of MRSA on hospital surfaces.
Received December 6, 2006; accepted February 19, 2007; electronically published June 11, 2007.
Cited by
. Infection Control and Hospital Epidemiology 30:6, 604-606Online publication date: 1-Jun-2009.
Online publication date: 1-Aug-2008.
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