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15 June 2007

Volume 195, Number 12
The Journal of Infectious Diseases 2007;195:1808–1817
© 2007 by the Infectious Diseases Society of America. All rights reserved.
0022-1899/2007/19512-0012$15.00
DOI: 10.1086/518007
MAJOR ARTICLE

Dengue Virus Infects Macrophages and Dendritic Cells in a Mouse Model of Infection

Jennifer L. Kyle,1,2

P. Robert Beatty,3 and

Eva Harris1,2

1Division of Infectious Diseases, School of Public Health, 2Graduate Group in Microbiology, and 3Department of Molecular and Cell Biology, University of California, Berkeley

Dengue fever is a mosquitoborne viral illness caused by 4 dengue viruses (DENV‐1–4). The cellular tropism of DENV has not been definitively determined, despite its importance for understanding viral pathogenesis and identifying therapeutic targets. To define DENV cellular tropism in a small animal model, 129/Pas mice lacking interferon‐α/β and/or ‐γ receptors were infected with DENV via a subcutaneous route. During the first week after infection, virus was present in lymph nodes, spleen, bone marrow, and circulating white blood cells. F4/80+CD11b+ macrophages and CD11c+ dendritic cells were demonstrated to be targets for DENV‐2 infection in the spleen by flow cytometry directed to structural and nonstructural DENV proteins and by magnetic bead separation followed by strand‐specific reverse‐transcriptase polymerase chain reaction. We find that the initial cellular tropism of DENV in mice is similar to that reported in humans, thereby paving the way for investigation of cellular tropism and pathogenesis of DENV in primary and secondary infections.

Received 20 November 2006; accepted 7 December 2006; electronically published 9 May 2007.

Reprints or correspondence: Eva Harris, Div. of Infectious Diseases, School of Public Health, University of California, Berkeley, 140 Warren Hall, Berkeley, CA 94720–7360 ().

  • Potential conflicts of interest: none reported.

    Presented in part: American Society of Tropical Medicine and Hygiene, Washington, D.C., 11–15 December 2005 (abstract 2005‐A‐110‐ASTMH); American Society for Virology, Montreal, Quebec, Canada, 10–14 July 2004 (abstract 00471).

    Financial support: Pediatric Dengue Vaccine Initiative (grant CRA‐14); Pacific Southwest Regional Centers for Excellence (project 1.2).

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