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1 October 2006

Volume 194, Number 7
The Journal of Infectious Diseases 2006;194:1003–1007
0022-1899/2006/19407-0018$15.00
DOI: 10.1086/507311
BRIEF REPORT

Chloroquine Prevents T Lymphocyte Suppression Induced by Anthrax Lethal Toxin

Mark I. Hirsh1 and

Victoria Cohen1,2

1Laboratory for Shock and Trauma Research, Rambam Medical Center, and 2Cancer and Vascular Biology Research Center, Ruth & Bruce Rappaport Faculty of Medicine, Technion, Haifa, Israel

Lysosomal processing of lethal toxin (LTX) is a key event in the pathogenesis of anthrax. This study investigated the ability of chloroquine (CQ) to interfere with this processing and thereby to reduce suppression of T lymphocytes. T lymphocytes isolated from blood were activated, by cross‐linking of CD3, in both the absence and presence of LTX and CQ and then were assayed by flow cytometry and immunoblotting. LTX was found to disrupt intracellular signaling, and it down‐regulated T lymphocyte function. CQ significantly reduced the harmful effects of LTX and protected the activation and cytokine production of T lymphocytes. This effect may indicate a promising strategy in the treatment of anthrax.

Received 14 March 2006; accepted 19 May 2006; electronically published 29 August 2006.

Reprints or correspondence: Dr. Mark I. Hirsh, Laboratory for Shock and Trauma Research, R. & B. Rappaport Faculty of Medicine, P.O.B. 9649, 31096 Haifa, Israel ().

Cited by

Mark I Hirsh, Irena Manov, Victoria Cohen-Kaplan, Theodore C Iancu. (2007) Ultrastructural features of lymphocyte suppression induced by anthrax lethal toxin and treated with chloroquine. Laboratory Investigation 87:2, 182-188
Online publication date: 1-Mar-2007.
CrossRef
  • Potential conflicts of interest: none reported.

    Financial support: NATO (grant LST.CLG 979523/02).

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