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15 April 2006

Volume 193, Number 8
The Journal of Infectious Diseases 2006;193:1089–1097
© 2006 by the Infectious Diseases Society of America. All rights reserved.
0022-1899/2006/19308-0006$15.00
DOI: 10.1086/501474
MAJOR ARTICLE

Identification of a Novel Single‐Stranded DNA Fragment Associated with Human Hepatitis

Chau‐Ting Yeh,

Mei‐Lin Tsao,

Ying‐Chun Lin, and

I‐Chu Tseng

Liver Research Unit, Chang Gung Medical Center, Taipei, Taiwan

By performing nonspecific polymerase chain reaction followed by elimination of chromosome‐derived sequences, foreign DNA fragments were obtained from the serum of a patient with non–A–E hepatitis. One of the sequences, named NV‐F, contained a partial open reading frame and was detected in 17 (24.6%) of 69 patients with non–A–E hepatitis, including 1 with fulminant hepatitis (vs. in 5 [2.8%] of 180 healthy individuals). A peptide was synthesized accordingly, to detect serum anti–NV‐F antibody, which was found in 49 (75.4%) of 65 patients positive for NV‐F. This DNA fragment was sensitive to S1 nuclease digestion. Cesium chloride gradient analysis revealed that the NV‐F–associated particles had buoyant densities of 1.33–1.39 and 1.22–1.25 g/mL. Immunofluorescence analysis revealed that the novel antigen was present in the hepatocytes of patients infected with NV‐F. In conclusion, we have identified a novel single‐stranded DNA fragment derived from a virus‐like agent associated with human hepatitis.

Received 28 September 2005; accepted 14 November 2005; electronically published 13 March 2006.

Reprints or correspondence: Dr. Chau‐Ting Yeh, Liver Research Unit, Chang Gung Medical Center, 199 Tung Hwa North Rd., Taipei, Taiwan ().

Cited by

Chau-Ting Yeh, Chao-Wei Hsu, Ming-Ling Chang, and Mei-Ling Tsao. (2008) Impact of the Novel Hepatotropic Viruslike Agent NV-F during Chronic Hepatitis C Virus Infection. The Journal of Infectious Diseases 198:12, 1742-1748
Online publication date: 15-Dec-2008.
Abstract-Full Text-PDF Version (398 kB)
Masahiko Kaito, Hideaki Tanaka, Shinichiro Horiike, Naoki Fujita, Motoh Iwasa, Yoshinao Kobayashi, Esteban Cesar Gabazza, Yukihiko Adachi, Masayoshi Konishi, Shozo Watanabe. (2007) Unidentified virus-like particles are detected in plasmas with elevated ALT levels: are they significant of etiological agent(s) of non-B, non-C hepatitis?. Medical Molecular Morphology 40:1, 23-28
Online publication date: 29-Apr-2007.
CrossRef
Eric L. Delwart. (2007) Viral metagenomics. Reviews in Medical Virology 17:2, 115-131
Online publication date: 1-Apr-2007.
CrossRef
Chau-Ting Yeh, Tse-Ching Chen, Ming-Ling Chang, Chao-Wei Hsu, Ta-Sen Yeh, Wei-Chen Lee, Shiu-Feng Huang, Chih-Chieh Tsai. (2007) Identification of NV-F virus DNA in hepatocellular carcinoma. Journal of Medical Virology 79:1, 92-96
Online publication date: 1-Feb-2007.
CrossRef

  • Potential conflicts of interest: none reported.

    Financial support: Chang Gung Medical Center (Chang Gung Medical Research Programs to the Molecular Medicine Research Center).

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