All Journals > The Journal of Infectious Diseases > 1 February 2005 > Clinical M. tuberculosis Strains in Macrophages

Article Tools

Search for Related Articles

Announcements

Science Watch logo

JID Article Named "New Hot Paper" by ScienceWatch.com

Dr. Lauri Hicks' 2007 article on pneumococcal disease has been named a "hot new paper" by Thompson Reuters' ScienceWatch.com. Read a Q&A about the article with Dr. Hicks here. 

Press Release

Unique Collaboration Charts the Migrations of a Parasite that Affected History
Researchers Sequence Louse DNA from Mummies and Propose New Model for its Development


In the News

Featured in Grist
"Another symptom of swine flu: instant amnesia" May 11, 2009
Swine Influenza Virus: Zoonotic Potential and Vaccination Strategies for the Control of Avian and Swine Influenzas
Eileen Thacker and Bruce Janke
Read the veterinary literature on swine flu and you get a strong sense of what might be called vaccination treadmill: the hog industry is literally scrambling to generate new vaccines for the rapidly evolving flu strains that sweep through CAFOs. Writing in the Journal of Infectious Diseases [PDF] in 2008, Eileen Thacker and Bruce Janke of Iowa State University paint a stark picture: “A number of genetically diverse viruses are circulating in swine herds throughout the world and are a major cause of concern to the swine industry,” they write. “Influenza virus infections in swine and poultry are potential sources of viruses for the next pandemic among humans.”

Featured in New York Times
"Fear of a Swine Flu Epidemic in 1976 Offers Some Lessons, and Concerns, Today" May 8, 2009
Anti‐Ganglioside Antibody Induction by Swine (A/NJ/1976/H1N1) and Other Influenza Vaccines: Insights into Vaccine‐Associated Guillain‐Barré Syndrome
Irving Nachamkin, Sean V. Shadomy, Anthony P. Moran, Nancy Cox, Collette Fitzgerald, Huong Ung, Adrian T. Corcoran, John K. Iskander, Lawrence B. Schonberger, and Robert T. Chen
Irving Nachamkin, a professor of pathology and laboratory medicine at the University of Pennsylvania, examined some 1976 vaccine that had been saved by a scientist in Texas. In a paper published last year in The Journal of Infectious Diseases, he and colleagues reported that mice given the vaccine made antibodies that reacted with gangliosides, which are components of nerve cells. An antibody attack on gangliosides is part of the disease mechanism of Guillain-Barré.

Featured in AFP
"Swine flu vaccine 'could be ready soon'" May 7, 2009
A Broadly Protective Vaccine against Globally Dispersed Clade 1 and Clade 2 H5N1 Influenza Viruses
Mary A. Hoelscher, Neetu Singh, Sanjay Garg, Lakshmi Jayashankar, Vic Veguilla, Aseem Pandey, Yumi Matsuoka, Jacqueline M. Katz, Ruben Donis, Suresh K. Mittal, and Suryaprakash Sambhara
The vaccine Mittal created for the bird flu worked on three different strains isolated over a seven-year period and was described in papers for the Journal of Infectious Diseases and the journal Clinical Pharmacology and Therapeutics.

Featured in Newsweek
"The Path of a Pandemic" http://www.newsweek.com/id/195692
Swine Influenza Virus: Zoonotic Potential and Vaccination Strategies for the Control of Avian and Swine Influenzas
Eileen Thacker and Bruce Janke
Last year researchers from Iowa State University in Ames warned that pigs located in industrial-scale farms were being subjected to influenza infections from farm poultry, wild birds and their human handlers. Writing in The Journal of Infectious Diseases, Eileen Thacker and Bruce Janke said, "As a result of the constantly changing genetic makeup of individual influenza viruses in pigs, the U.S. swine industry is continually scrambling to respond to the influenza viruses circulating within individual production systems."

1 February 2005

Volume 191, Number 3
The Journal of Infectious Diseases 2005;191:453–460
© 2004 by the Infectious Diseases Society of America. All rights reserved.
0022-1899/2005/19103-0017$15.00
DOI: 10.1086/425936
MAJOR ARTICLE

Intracellular Macrophage Growth Rates and Cytokine Profiles of Mycobacterium tuberculosis Strains with Different Transmission Dynamics

Sue A. Theus,1

M. Donald Cave,2 and

Kathleen D. Eisenach1,3

1Departments of Pathology, 2Neurobiology and Developmental Science, and 3Microbiology‐Immunology, Central Arkansas Veterans Healthcare System, University of Arkansas for Medical Sciences, Little Rock

Mycobacterium tuberculosis strains associated with IS6110 restriction fragment–length polymorphism (RFLP) pattern clusters and strains demonstrating unique IS6110 RFLP patterns were investigated in interferon‐γ–activated THP‐1 cells by measurement of binding, intracellular growth rate, and cytokine production. Binding was the same for all strains; however, strains from clusters grew significantly more rapidly than did unique strains. Maximal concentration of tumor necrosis factor (TNF)–α was detected at 2 days after infection, with unique strains eliciting significantly greater amounts than did strains from clusters. Interleukin (IL)–10 levels peaked at 1 day after infection with strains from clusters, whereas they peaked at 5 days after infection with unique strains. Rapid growth demonstrated by strains from clusters was highly correlated with rapid production of IL‐10 and suppression of TNF‐α in THP‐1 cells during the early stages of infection. Characterization of this phenotype will further advance the investigation of virulence factors in M. tuberculosis.

Received 13 April 2004; accepted 24 June 2004; electronically published 30 December 2004.

Reprints or correspondence: Dr. Kathleen D. Eisenach, Central Arkansas Veterans Healthcare System, Medical Research Services, 151/LR, 4300 W. 7th St., Rm. GB‐126, Little Rock, AR 72205 ().

Cited by

John W. Theus, Robert S. Justus, Sue A. Theus. (2009) A correlation between growth rate, apoptosis, and tumor necrosis factor-α in umbilical cord blood cells infected with two strains of Mycobacterium tuberculosis. Transfusion
Online publication date: 1-Jun-2009.
CrossRef
S. Wu, P. F. Barnes, B. Samten, X. Pang, S. Rodrigue, S. Ghanny, P. Soteropoulos, L. Gaudreau, S. T. Howard. (2009) Activation of the eis gene in a W-Beijing strain of Mycobacterium tuberculosis correlates with increased SigA levels and enhanced intracellular growth. Microbiology 155:4, 1272-1281
Online publication date: 1-May-2009.
CrossRef
H. Sohn, K.-S. Lee, S.-Y. Kim, D.-M. Shin, S.-J. Shin, E.-K. Jo, J.-K. Park, H.-J. Kim. (2009) Induction of Cell Death in Human Macrophages by a Highly Virulent Korean Isolate of Mycobacterium tuberculosis and the Virulent Strain H37Rv. Scandinavian Journal of Immunology 69:1, 43-50
Online publication date: 1-Feb-2009.
CrossRef
A. Martín, F. Chaves, J. Iñigo, M. Alonso, C. Sola, N. Rastogi, M. J. Ruiz Serrano, E. Palenque, E. Bouza, D. García de Viedma. (2008) Molecular, epidemiological and infectivity characterisation of a Mycobacterium tuberculosis strain prevalent in Madrid. Clinical Microbiology and Infection 13:12, 1210-1213
Online publication date: 1-Jan-2008.
CrossRef
Elisabetta Volpe, Giulia Cappelli, Manuela Grassi, Angelo Martino, Annalucia Serafino, Vittorio Colizzi, Nunzia Sanarico, Francesca Mariani. (2006) Gene expression profiling of human macrophages at late time of infection with Mycobacterium tuberculosis. Immunology 0:0, 060710044926002-???
Online publication date: 10-Aug-2006.
CrossRef
David W. Dunne, Anne Cooke. (2005) Opinion: A worm's eye view of the immune system: consequences for evolution of human autoimmune disease. Nature Reviews Immunology 5:5, 420-426
Online publication date: 1-Jun-2005.
CrossRef

  • Financial support: National Institutes of Health (grant N01 AI95383).

Back to Top
Close Popup