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1 February 2004

Volume 189, Number 3
The Journal of Infectious Diseases 2004;189:431–439
0022-1899/2004/18903-0010
DOI: 10.1086/381166
MAJOR ARTICLE

Susceptibility of Common Fibroblast Cell Lines to Transmissible Spongiform Encephalopathy Agents

Ina Vorberg,1

Anne Raines,1

Brian Story,2 and

Suzette A. Priola1

1Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana; 2Department of Laboratory Medicine, University of Washington, Seattle, Washington

The risk of contamination of tissue culture cells with transmissible spongiform encephalopathy (TSE) agents as a result of the use of animal products as medium components has been considered to be low, in part, because only a few brain‐derived cell lines have been reported to be susceptible to TSE infection. In the present study, we demonstrate that the common laboratory fibroblast cell lines NIH/3T3 and L929, which express low levels of cellular mouse prion protein, are susceptible to infection with mouse‐adapted scrapie. Our results show that the susceptibility of a cell line to TSE infection cannot be predicted on the basis of its tissue origin or its level of expression of the cellular prion protein, and they suggest that any cell line expressing normal host prion protein could have the potential to support propagation of TSE agents. Thus, testing of cells for TSE susceptibility might be necessary for all cell lines that are routinely used in vaccine production and in other medical applications.

Received 29 April 2003; accepted 14 August 2003; electronically published 21 January 2004.

Reprints or correspondence: Dr. Ina Vorberg, Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 S. 4th St., Hamilton, MT 59840 ().

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