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01 May 1999 Supplement

Volume 179, Number S3
The Journal of Infectious Diseases 1999;179:S448–S453
0022-1899/99/79S3-0015$02.00
DOI: 10.1086/314802

Infectious Human Immunodeficiency Virus Can Rapidly Penetrate a Tight Human Epithelial Barrier by Transcytosis in a Process Impaired by Mucosal Immunoglobulins

Hakim Hocini and

Morgane Bomsel

U.322‐Institut Cochin de Génétique Moléculaire, Paris, France

Mucosal surfaces are the main natural site of entry into the body for human immunodeficiency virus (HIV). Herein, an alternative mechanism for virus spread is described. The mechanism, which involves transcytosis of endosome‐internalized HIV‐particles, was generated by contact of HIV‐infected cells with the apical surface of an epithelial cell line. Transcytosed viruses rapidly (in 20–30 min) access the serosal side of the epithelial barrier without infecting the epithelium itself. In turn, transcytosed HIV could infect host submucosal mononucleated target cells, and thus the infection could spread. An investigation was done to determine whether mucosal antibodies could block HIV transcytosis. Both secretory IgA (S‐IgA) and IgG that were purified from colostrum from HIV‐seropositive women impaired HIV transcytosis, irrespective of the level of the recombinant HIV envelope anti‐gp160–specific activities in an ELISA. However, specific S‐IgAs were more efficient than IgG. Therefore, mucosal‐specific S‐IgA to HIV‐1 could be relevant to reducing infectivity of HIV‐1 in corporeal fluids.

Reprints or correspondence: Dr. Morgane Bomsel, U. 332‐Institut Cochin de Génétique Moléculaire, 22, rue Méchain F‐75014, Paris, France ().

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